Resveratrol Protects PC12 Cell against 6-OHDA Damage via CXCR4 Signaling Pathway

Figure 4

Effects of 6-OHDA and resveratrol on MMP of PC12 cells. (a–c) The fluorescent images of PC12 cells stained with JC-1 dye and captured by a Zeiss 780 laser confocal microscope. In the normal control group, cells exhibited clearly orange red (a), while, in 6-OHDA injury group, quite amounts of cells had high level of green fluorescent intensity, with lower level of red fluorescence (b). When preincubated with resveratrol, the green fluorescent intensity was effectively decreased meanwhile red fluorescence was increased (c). (d) Quantification of fluorescent intensity of PC12 cells in different group. Statistical analysis showed that the red/green fluorescence intensity ratio of PC12 cells treated with 6-OHDA was obviously decreased, which could be prevented by 25 μM resveratrol. Ratio of red/green fluorescence was presented as means ± SD from five independent experiments. versus control group, versus 6-OHDA injury group. Scale bars: (a–c) 50 μm.

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