Resveratrol Protects PC12 Cell against 6-OHDA Damage via CXCR4 Signaling Pathway

Figure 5

Resveratrol downregulates the expression of CXCR4 in PC12 cells treated with 6-OHDA. Immunofluorescent staining (a–d) and Western blotting (e, f) were used to determine the expression of CXCR4. (a) In normal control group, the intensity of the staining was weak. After being treated with 6-OHDA, the fluorescent intensity was increased (b). In the presence of resveratrol, this increase was effectively decreased (c). Arrows (a, b, and c) indicated the positive fluorescent staining. (d) Quantification of fluorescent intensity of PC12 cells in different group. In Western blotting, the level of CXCR4 protein was significantly increased in cells treated with 6-OHDA and preincubation with resveratrol could decrease the upregulated protein levels of CXCR4 (e and f). Values of fluorescent intensity were presented as means ± SD from five independent experiments. versus control group, versus 6-OHDA injury group. Scale bars: (a–c) 100 μm.

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