Antinociceptive and Anti-Inflammatory Effects of Total Alkaloid Extract from Fumaria capreolata

Table 1

Primer sequences used in RT-PCR assays in RAW264.7 cells.

Gene	Primers sequences	Annealing (°C)
IL-6	FW 5′-CTTCCCTACTTCACAAGTC-3′	60
	RV 5′-CTCCATTAGGAGAGCATTG-3′
TNF-α	FW 5′-AACTAGTGGTGCCAGCCGAT-3′	56
	RV 5′-CTTCACAGAGCAATGACTCC-3′
iNOS	FW 5′-GTTGAAGACTGAGACTCTGG-3′	56
	RV 5′-GACTAGGCTACTCCGTGGA-3′
IL-1β	FW 5′-TGATGAGAATGACCTCTTCT-	55
	RV 5′-CTTCTTCAAAGATGAAGGAAA-3′
COX-2	FW 5′-GGGTTGCTGGGGGAAGAAATG-3′	67
	RV 5′-GGTGGCTGTTTTGGTAGGCTG-3′
MIP-2	FW 5′-CAGTTAGCCTTGCCTTTGTTCAG-3′	62
	RV 5′-CAGTGAGCTGCGCTGTCCAATG-3′
GADPH	FW 5′-CCATCACCATCTTCCAGGAG-3′	60
	RV 5′-CCTGCTTCACCACCTTCTTG-3′