Hypoglycemic Effect of Aqueous and Methanolic Extract of Artemisia afra on Alloxan Induced Diabetic Swiss Albino Mice

Table 3

Results of preliminary phytochemical analysis in aqueous crude extracts of Artemisia afra aerial part.

Secondary metabolite	Reagents and method	Indicators	Artemisia afra
Chromophores	10 mL distilled water + heat for 30 min	Yellow to red color	+++
Polyphenols	1% FeCl3 + 1 mL of K3Fe (CN)6	Green blue color	− − −
Saponins	30 mL distilled water + heat (5 min)	Formation of honey comb froth	+++
Phytosteroids and withanoids	CHCl3 + conc. H2SO4	Red, reddish brown, or violet color	+++
Flavonoids	5 drops of 2% lead acetate	Yellow or orange color	+++
Tannins	3 drops of 1% K3[Fe(CN)6] + 3 drops of conc. NH3	Formation of color	+++
Anthraquinone glycosides	2 N HCL, benzene, 10% ammonia	Red color	+++