Synergistic Use of Geniposide and Ginsenoside Rg1 Balance Microglial TNF- and TGF-1 following Oxygen-Glucose Deprivation In Vitro: A Genome-Wide Survey

Figure 2

(a) Expression of TNF-α and TGF-β1 in microglia was assessed by western blotting. Photographs show representative blots of TNF-α, TGF-β1, and β-actin (loading control). (b) Bar graphs show the relative densities of TNF-α and TGF-β1 bands on western blotting estimated quantitatively by Phoretix 1D image software. Values represent the mean optical density ratio relative to the loading control. (c) Bar graphs show the contents of TNF-α and TGF-β1 in the cultured media. (d) Immunofluorescence for TNF-α in different treated groups. versus control, versus control, versus model, and versus model. Control, normal cultured microglial cells; Model, OGD-injured microglial cells; Gen, geniposide-treated microglial cells; Rg1, ginsenoside-Rg1-treated microglial cells; Gen+Rg1, combination-treated microglial cells.

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