Inhibitory Effects of Cytosolic Ca²⁺ Concentration by Ginsenoside Ro Are Dependent on Phosphorylation of IP₃RI and Dephosphorylation of ERK in Human Platelets

Figure 7

Effects of G-Ro on dephosphorylation of ERK. Lane 1, unstimulated platelets (base); Lane 2, thrombin (0.05 U/mL); Lane 3, thrombin (0.05 U/mL) + G-Ro (50 μM); Lane 4, thrombin (0.05 U/mL) + G-Ro (100 μM); Lane 5, thrombin (0.05 U/mL) + G-Ro (200 μM); Lane 6, thrombin (0.05 U/mL) + G-Ro (300 μM). Western blotting was performed as described in “Section 2.” The data are expressed as the mean ± standard deviation (). versus the thrombin-stimulated human platelets.