Inhibitory Effects of Cytosolic Ca²⁺ Concentration by Ginsenoside Ro Are Dependent on Phosphorylation of IP₃RI and Dephosphorylation of ERK in Human Platelets

Figure 8

Effects of G-Ro on ATP and serotonin release. (a) Effects of G-Ro on ATP release in thrombin-activated platelets. (b) Effects of G-Ro on serotonin release in thrombin-activated platelets. (c) Effects of G-Ro on ATP release in the presence of A-kinase inhibitor (Rp-8-Br-cAMPS) and G-kinase inhibitor (Rp-8-Br-cGMPS). (d) Effects of G-Ro on serotonin release in the presence of A-kinase inhibitor (Rp-8-Br-cAMPS) and G-kinase inhibitor (Rp-8-Br-cGMPS). Determination of ATP and serotonin release was carried out as described in “Section 2.” The data are expressed as mean ± standard deviation (). versus the thrombin-stimulated human platelets, versus the thrombin-stimulated human platelets in the presence of G-Ro (155 μM), and versus the thrombin-stimulated human platelets in the presence of G-Ro (300 μM).

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