PMC-12, a Prescription of Traditional Korean Medicine, Improves Amyloid -Induced Cognitive Deficits through Modulation of Neuroinflammation

Figure 5

Effects of PMC-12 on MAPKs activation induced by LPS in microglia. BV2 cells were treated with the indicated dose of PMC-12 (1, 3, 10, or 30 μg/mL) 1 h before LPS treatment (1 μg/mL) for 24 h. Total protein (50 μg) was subjected to 8% SDS-PAGE, followed by Western blotting using anti-p38, anti-ERK-1/2, and anti-JNK. PMC-12 significantly inhibited p38, ERK-1/2, and JNK MAPK activation in a dose-dependent manner. Results are representative of those obtained from four independent experiments. Actin was used as an internal control.