UP3005, a Botanical Composition Containing Two Standardized Extracts of Uncaria gambir and Morus alba, Improves Pain Sensitivity and Cartilage Degradations in Monosodium Iodoacetate-Induced Rat OA Disease Model

Table 3

Modified Mankin scoring system for histopathological findings for MIA-induced rats treated with UP3005 orally.

Group/dose	Structure; (0~6)	Cellular abnormality; (0~3)	Safranin O staining; (0~4)	Total Mankin score
Normal	0	0	0	0
Vehicle	2.78 ± 1.79	1.78 ± 0.44	2.67 ± 1.32	7.22 ± 3.19
Diclofenac (10 mg/kg)	1.9 ± 1.45	1.3 ± 0.67	1.7 ± 0.82	4.9 ± 2.69
UP3005 (500 mg/kg)		1.4 ± 0.52	1.3 ± 0.9	3.7 ± 1.3

Structure (0~6)—0 = normal, 1 = irregular surface, including fissures into the radial layer, 2 = pannus, 3 = absence of superficial cartilage layers, 4 = slight disorganization (an absent cellular row and some small superficial clusters), 5 = fissures into the calcified cartilage layer, and 6 = disorganization (chaotic structure, clusters, and osteoclastic activity); cellular abnormalities (0~3)—0 = normal, 1 = hypercellularity, including small superficial clusters, 2 = clusters, and 3 = hypocellularity; matrix staining (0~4)—0 = normal/slight reduction of staining, 1 = staining reduced in the radial layer, 2 = staining reduced in the interterritorial matrix, 3 = staining reduced in pericellular matrix, and 4 = staining absent. = 10 rats/group, versus vehicle.