Aortic Remodelling Is Improved by 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside Involving the Smad3 Pathway in Spontaneously Hypertensive Rats

Figure 4

Effect of THSG on Smad3 deacetylation and DNA binding ability in vivo. (a) Acetylation of Smad3 detected using immunoprecipitation and Western blot assays in aortas from WKY rats, SHRs, and SHRs treated with THSG (). Data are expressed as the mean ± SEM. versus WKY; versus SHRs. (b) Smad3 binding activation was determined using a chromatin immunoprecipitation assay on promoter proximal, remote, and exon 2 regions of rat PAI-1 gene in aortas from WKY rats, SHRs, and SHRs treated with THSG. Bottom panel: Smad3 binding relative densities in rat PAI-1 proximal promoter were analyzed ().

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