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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 794729, 7 pages
Research Article

Bioactive Polyphenols from the Methanol Extract of Cnicus arvensis (L.) Roth Demonstrated Antinociceptive and Central Nervous System Depressant Activities in Mice

1Natural Product Chemistry and Pharmacology Research Laboratory, Faculty of Health Sciences, Department of Pharmacy, Northern University Bangladesh, Dhaka 1205, Bangladesh
2Phytochemistry and Pharmacology Research Laboratory, Department of Pharmacy, Manarat International University, Dhaka 1216, Bangladesh
3Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh
4Chemical Research Division, BCSIR Laboratories, Bangladesh Council of Scientific & Industrial Research, Dhaka 1205, Bangladesh

Received 25 November 2014; Revised 25 December 2014; Accepted 29 December 2014

Academic Editor: Olumayokun A. Olajide

Copyright © 2015 Mahmudur Rahman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cnicus arvensis is used by many ethnic groups for inflammation, pain, and other ailments. In this study, reducing sugar, carbohydrate, alkaloid, steroid, tannin, flavonoid, and saponin groups were identified using standard chromogenic method. In high-performance liquid chromatography, vanillic acid and epicatechin were identified in the extract. Antinociceptive test by acetic acid induced writhing inhibition resulted 43.17 and 95.08% inhibition for 100 and 200 mg/kg body weight, comparing with standard diclofenac Na with 74.86% inhibition for 25 mg/kg body weight. In formalin induced paw licking test for antinociceptive activity, the extract inhibited 69.87 and 75.55% licking for 150 and 300 mg/kg body weight comparing with the inhibition (68.56%) of diclofenac Na for 10 mg/kg body weight at first phase. At late phase, the extract showed 73.12 and 87.46% licking comparing with licking inhibition (71.69%) by diclofenac Na at the same dose. In open field test for CNS depressant activity, the extract showed depression of locomotor activity for 150 and 300 mg/kg body weight comparing with diazepam for 10 mg/kg body weight. All results were statistically significant . The identified polyphenols are reputed for antinociceptive and CNS depressant activity. The present findings support the use of this plant in pain.