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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 801691, 11 pages
http://dx.doi.org/10.1155/2015/801691
Research Article

1H NMR Metabolic Profiling of Biofluids from Rats with Gastric Mucosal Lesion and Electroacupuncture Treatment

1Department of Electronic Science and Department of Traditional Chinese Medicine, Xiamen University, Xiamen 361005, China
2Department of Bioprocess Engineering and Innovation Centre in Agritechnology, Universiti Teknologi Malaysia, 81310 Johor Bahru, Malaysia
3College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410000, China

Received 11 February 2015; Accepted 3 April 2015

Academic Editor: Shuhai Lin

Copyright © 2015 Jingjing Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Gastric mucosal lesion (GML) is a common gastrointestinal disorder with multiple pathogenic mechanisms in clinical practice. In traditional Chinese medicine (TCM), electroacupuncture (EA) treatment has been proven as an effective therapy for GML, although the underlying healing mechanism is not yet clear. Here, we used proton nuclear magnetic resonance- (1H NMR-) based metabolomic method to investigate the metabolic perturbation induced by GML and the therapeutic effect of EA treatment on stomach meridian (SM) acupoints. Clear metabolic differences were observed between GML and control groups, and related metabolic pathways were discussed by means of online metabolic network analysis toolbox. By comparing the endogenous metabolites from GML and GML-SM groups, the disturbed pathways were partly recovered towards healthy state via EA treated on SM acupoints. Further comparison of the metabolic variations induced by EA stimulated on SM and the control gallbladder meridian (GM) acupoints showed a quite similar metabolite composition except for increased phenylacetylglycine, 3,4-dihydroxymandelate, and meta-hydroxyphenylacetate and decreased N-methylnicotinamide in urine from rats with EA treated on SM acupoints. The current study showed the potential application of metabolomics in providing further insight into the molecular mechanism of acupuncture.