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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 810369, 13 pages
http://dx.doi.org/10.1155/2015/810369
Research Article

Hugan Qingzhi Exerts Anti-Inflammatory Effects in a Rat Model of Nonalcoholic Fatty Liver Disease

1Center for Drug Research and Development, Zhujiang Hospital, Southern Medical University, Guangdong, Guangzhou 510282, China
2Department of Pharmacy, Ganzhou People’s Hospital, Nanchang University, Jiangxi, Ganzhou 341000, China
3Department of Pharmacy, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China
4Department of Pharmacy, Beijiao Hospital, Southern Medical University, Guangdong, Guangzhou 528311, China
5Department of Traditional Chinese Medicine, Zhujiang Hospital, Southern Medical University, Guangdong, Guangzhou 510282, China

Received 20 January 2015; Revised 14 May 2015; Accepted 18 May 2015

Academic Editor: Yoshiji Ohta

Copyright © 2015 WaiJiao Tang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ethnopharmacological Relevance. The Hugan Qingzhi tablet (HQT) is a traditional Chinese medicine used for treating NAFLD (nonalcoholic fatty liver disease). The present study evaluated the anti-inflammatory effects of HQT in rats with NAFLD. Materials and Methods. HQT was administered daily to the NAFLD experimental groups. Biochemical markers, histopathological data, and oxidative stress/antioxidant biomarkers were determined. Proinflammatory cytokines interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), and interleukin-6 (IL-6) were detected by enzyme-linked immunoassay. Expressions of silent information regulator 1 (SIRT1) and acetylated-nuclear-factor kappaB-p65 (Ac-NF-κB-p65) were performed by western blotting. Results. At high and moderate doses, HQT was highly effective in decreasing serum alanine aminotransferase (), aspartate aminotransferase (), hepatic total cholesterol (), triglycerides (), and free fatty acid levels (). Moreover, high and moderate doses of HQT reduced hepatic levels of the proinflammatory cytokines TNF-α (), IL-1β (), and IL-6 (), enhanced SIRT1 expression, and depressed Ac-NF-κB-p65 expression at protein level. Conclusions. In our NAFLD rat model, HQT exerted substantial anti-inflammatory and antioxidant activities, possibly involving the regulation of SIRT1 and Ac-NF-κB-p65 expression.