Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis

Figure 2

Orthotopic models in vivo and stable high level expression of GFP in gastric cancer metastatic tumors in nude mice. (a) and (b) 5 × 10⁶ MKN-45-GFP cells were inoculated subcutaneously into the left flank of 5–7-week-old anesthetized nude mice. 6 weeks later, subcutaneous tumor grew obviously. (c) Subcutaneous tumor was harvested and incised into small fragments. (d) and (e) Tumor pieces were orthotopically implanted in the gastric wall with surgical suture. (f) and (g) Under the LZ12 microscope, gastric orthotopic tumors expressed strong GFP fluorescence. (h) GFP signals from subcutaneous tumor pieces were tested under fluorescence microscopy. (i) 6 to 8 weeks after the orthotopic implantation, gastric metastasis was investigated. Detection of GFP signal confirmed the metastatic tumors (arrows) and microtumors or gastric cancer cells (arrowheads).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)