Neuroprotective Activity of Coptisine from Coptis chinensis (Franch)

Figure 4

Effect of CRE (100 μg/mL) and Cop (20 μM) on DCF fluorescence was investigated in SH-SY5Y cells. Increase in DCF fluorescence indicates increased intracellular ROS. (a) shows the evolution of ROS generation; t-BOOH (upper 4 lines) or PBS (lower 4 lines) was added at the start of the measurements. Results are expressed as mean DCF fluorescence ± SEM normalized to the medium control of three independent experiments which were conducted in triplicate. (b) represents the mean sum of the DCF fluorescence signal normalized to the medium control, which was set to 100%. Note that only pretreatment with CRE significantly reduces mean DCF fluorescence. versus control. versus Cop.

(a)

(b)