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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 828437, 9 pages
Research Article

Validation of a RP-HPLC-DAD Method for Chamomile (Matricaria recutita) Preparations and Assessment of the Marker, Apigenin-7-glucoside, Safety and Anti-Inflammatory Effect

1Laboratório de Pesquisa, Desenvolvimento e Inovação (P, D & I), Apis Flora Industrial e Comercial LTDA, 14020-670 Ribeirão Preto, SP, Brazil
2Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), 14055-370 Ribeirão Preto, SP, Brazil
3Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), 14040-903 Ribeirão Preto, SP, Brazil
4Department of Pharmacology, School of Medicine of Ribeirao Preto, University of Sao Paulo, 14055-370 Ribeirao Preto, SP, Brazil
5Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), 14040-903 Ribeirão Preto, SP, Brazil

Received 21 May 2015; Revised 14 August 2015; Accepted 19 August 2015

Academic Editor: G. K. Jayaprakasha

Copyright © 2015 Felipe Galeti Miguel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Chamomile is a medicinal plant, which presents several biological effects, especially the anti-inflammatory effect. One of the compounds related to this effect is apigenin, a flavonoid that is mostly found in its glycosylated form, apigenin-7-glucoside (APG), in natural sources. However, the affectivity and safety of this glycoside have not been well explored for topical application. In this context, the aim of this work was to develop and validate a reversed-phase high-performance liquid chromatography (RP-HPLC-DAD) method to quantify APG in chamomile preparations. Additionally, the safety and the anti-inflammatory potential of this flavonoid were verified. The RP-HPLC-DAD method was developed and validated with linearity at 24.0–36.0 μg/mL range (). Intra- and interday precision (RSD) were 0.27–2.66% and accuracy was 98.27–101.21%. The validated method was applied in the analysis of chamomile flower heads, glycolic extract, and Kamillen cream, supporting the method application in the quality control of chamomile preparations. Furthermore, the APG safety was assessed by MTT cytotoxicity assay and mutagenic protocols and the anti-inflammatory activity was confirmed by a diminished TNF- production showed by mice macrophages treated with APG following LPS treatment.