Gigantol Suppresses Cancer Stem Cell-Like Phenotypes in Lung Cancer Cells

Figure 3

Gigantol suppresses CSC-like phenotypes. (a) After being treated with gigantol (0–20 µM) for 48 h, H460 cells were suspended and subjected to spheroid formation assay. (b) 4x phase-contrast images of primary spheroids at day 7 were captured for treated and nontreated cells. (c) The primary spheroids were resuspended into single cells, and secondary spheroids were allowed to grow for 30 days. (d) 4x phase-contrast images of secondary spheroids at day 30 were presented. All plots are means ± SD ( = 3). < 0.05 versus nontreated cells.

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