Chemical Constituents and Evaluation of Antimicrobial and Cytotoxic Activities of Kielmeyera coriacea Mart. & Zucc. Essential Oils

Table 2

Inhibitory effect against aerobic and anaerobic oral bacteria and cytotoxic activity (CC₅₀, μg mL⁻¹) of essential oils from K. coriacea.

		Minimum inhibitory concentration (MIC) – μg mL^−1
Microorganisms		Samples of essential oils				CHD^*
		Leaves	Inner bark	Outer bark	Wood
Anaerobic	Actinomyces naeslundii^b; ATCC 19039	>400	>400	400	>400	1.844
	Bacteroides fragilis^a; ATCC 25285	>400	>400	400	>400	1.844
	Prevotella nigrescens^a; ATCC 33563	200	50	100	200	1.844
Aerobic	Streptococcus mitis^b; ATCC 49456	>400	100	100	100	3.688
	Streptococcus mutans^b; ATCC 25175	>400	200	100	100	0.922
	Streptococcus sanguinis^b; ATCC 10556	>400	400	100	100	0.922
	Streptococcus sobrinus^b; ATCC 33478	>400	100	100	100	0.922
Cytotoxic activity (CC50, μg mL^−1)
		Leaves	Inner bark	Outer bark		Wood
Vero cells  ATCC CCL 81		168 ± 10	74 ± 3	127 ± 1		>512
Selectivity index for Anaerobic microorganisms		−0.38; −0.38; −0.076	−0.73; −0.73; 0.17	−0.50; −0.50; 0.10		0.11; 0.11; 0.41
Selectivity index for Aerobic microorganisms		−0.38	−0.13, −0.43; −0.73; −0.13	0.10		0.71

Gram-negative bacteria; ^bGram-positive bacteria; ^*CHD: chlorhexidine dihydrochloride (positive control).