Gambogic Acid Lysinate Induces Apoptosis in Breast Cancer MCF-7 Cells by Increasing Reactive Oxygen Species

Figure 3

Gambogic acid lysinate can increase intracellular ROS level and NAC can block it. Exponential growth MCF-7 cells were treated with PBS (negative control), Rosup 100 mg/L (positive control), gambogic acid lysinate 2μmol/L, NAC 2 mmol/L, and gambogic acid lysinate 2μmol/L + NAC 2 mmol/L for 24 h. After treatment MCF-7 cells were stained with DCFH-DA and were determined by fluorescence microscopy (a, b) and fluorescence spectrophotometer (c).

(a)

(b)

(c)