Relationship of Genetic Polymorphisms of the Chemokine, CCL5, and Its Receptor, CCR5, with Coronary Artery Disease in Taiwan

Figure 1

Polymerase chain reaction-restriction fragment length polymorphisms of CCL5-403 and CCR5-59029 genes. (a) PCR products of CCL5-403 gene polymorphisms were subjected to enzymatic digestion by incubation with Rsa I, for 4 hours at 37°C, and then submitted to electrophoresis in 3% agarose gels. The wild homozygous alleles (C/C) yielded a 206-bp product, and the heterozygous alleles (C/T) yielded 206-, 180-, and 26-bp products, while the mutant homozygous alleles (T/T) yielded 180- and 26-bp products. (b) PCR products of the CCR5-59029 gene polymorphism were subjected to enzymatic digestion by incubation with Bsp 1286I. The wild homozygous alleles (G/G) yielded 134-bp products, and the heterozygous alleles (G/A) yielded 268- and 134-bp products, while the mutant homozygous alleles (A/A) yielded a 268-bp product.

(a) CCL5-403

(b) CCR5-59029