Research Article

Effect of Eugenol against Streptococcus agalactiae and Synergistic Interaction with Biologically Produced Silver Nanoparticles

Figure 1

Effect of eugenol on growth (a–c) and viability (d-e) of Streptococcus agalactiae. Time-kill curves of GBS 89 (a), GBS 121 (b), and S. agalactiae ATCC 13813 (c) strains: bacteria were incubated with eugenol at MIC for 24 h at 37°C and the CFU counts were determined at specified time points. Viability of the cells was determined with live-dead staining and GBSs with intact membranes were green-fluorescent (d), whereas eugenol-treated GBSs (at MIC) with damaged membranes were red-fluorescent (e). Representative images are shown. Bar = 5 μm.