Chronic Treatment with a Water-Soluble Extract from the Culture Medium of Ganoderma lucidum Mycelia Prevents Apoptosis and Necroptosis in Hypoxia/Ischemia-Induced Injury of Type 2 Diabetic Mouse Brain

Figure 4

Effects of chronic pretreatment with MAK (1 g/kg) on apoptotic cell death in the ischemic penumbral regions. Representative photographs of TUNEL staining and cleaved caspase-3 immunostaining at 24 h of reoxygenation after H/I in the penumbral cortex (a), (e) and hippocampal CA1 region (c), (g) from the mice in each group. Scale bar = 50 μm. Quantification of the number of TUNEL-positive (arrow heads) or cleaved caspase-3-positive cells was achieved by cell counting in the penumbral cortex (b), (f) and hippocampal CA1 region (d), (h). Throughout, data are represented as means ± S.D. . compared with the respective sham-operated groups. , 0.01 compared with the H/I-treated control group.