Research Article

Evaluation of Sedative and Hypnotic Activity of Ethanolic Extract of Scoparia dulcis Linn.

Table 6

Phytochemicals identified in EESD.

PhytochemicalsName of the testsObserved changesResult

AlkaloidsMayer’s testYellowish buff color precipitate+
Hager’s testYellow crystalline precipitate
Wagner’s testBrown or deep brown precipitate
Dragendorff’s testOrange or orange-brown precipitate+
Tannic acid testBuff color precipitate

GlycosidesGeneral testYellow color+
Test for glucosideProduction of brick-red precipitation+

FlavonoidsHydrochloric acid reduction testRed color+

CarbohydratesMolisch’s testA red or reddish violet ring is formed at the junction of two layers and on shaking a dark purple solution is formed+
Barfoed’s test (general test for monosaccharides)Red precipitate
Fehling’s testA red or brick-red precipitate+
Benedict’s test
Test for reducing sugarA brick-red precipitate+

SaponinsFrothing testFormation of stable foam+

SteroidsSulphuric acid testRed color+

Tannins
 
Ferric chloride test Blue green color
Alkaline reagent testYellow to red precipitate+