Trigonella foenum-graceum (Seed) Extract Interferes with Quorum Sensing Regulated Traits and Biofilm Formation in the Strains of Pseudomonas aeruginosa and Aeromonas hydrophila

Table 4

Effect of sub-MICs of methanolic extract of Trigonella foenum-graceum (seed) on inhibition of quorum sensing regulated virulence factorsin Aeromonas hydrophila WAF-38.

Concentration (g/mL)	Total protease^a	EPS production^b	Biofilm formation^c
Control	0.589 ± 0.051	0.748 ± 0.021	0.226 ± 0.006
100	0.520 ± 0.025; (11.7)	0.721 ± 0.064; (03.6)	0.126 ± 0.011; (44.2)^*
200	0.431 ± 0.049; (26.8)	0.642 ± 0.035; (14.1)	0.077 ± 0.018; (65.9)^**
400	0.321 ± 0.033; (45.5)^*	0.516 ± 0.013; (31.0)	0.068 ± 0.004; (69.9)^**
800	0.167 ± 0.018; (71.6)^**	0.401 ± 0.021; (46.3)^*	0.052 ± 0.010; (76.9)^**

Total protease activity is expressed as the absorbance at OD₆₀₀.
^bEPS production is expressed as absorbance at OD₄₈₀.
^cBiofilm formation is expressed as OD₄₇₀ after incubation with crystal violet.
The data represents mean values of three independent experiments. ^*Significance at , ^**significance at .
Values in the parentheses indicate percent reduction over control.