Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 1

Research Article

Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK1/2 in 3T3-L1 Cells

Figure 1

Cytotoxic effects of SSE extract in undifferentiated and differentiated 3T3-L1 cells. (a) 3T3-L1 preadipocytes were treated with various concentrations of SSE (0, 31.25, 62.5, 125, 250, or 500 μg/mL) for 24 h. (b) 3T3-L1 preadipocytes were differentiated into adipocytes by incubation with isobutylmethylxanthine, dexamethasone, and insulin (MDI) for 8 days. The cells were exposed to various concentrations of SSE (0, 62.5, 125, 250, 500, or 1000 μg/mL) during the differentiation period. Cell viability was determined using a CCK-8 assay kit by measuring the absorbance at 450 nm. Data are presented as mean SEM.