Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 3

Research Article

Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK1/2 in 3T3-L1 Cells

Figure 3

Inhibitory effects of SSE on GPDH activity and leptin production in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes by incubation with isobutylmethylxanthine, dexamethasone, and insulin (MDI) for 8 days. The cells were exposed to various concentrations of SSE (0, 25, 50, 100, 200, or 400 μg/mL) during the differentiation period. (a) GPDH activity of the cells was assessed by measuring the decrease in NADH at 340 nm using a TAKARA glycerol-3-phosphate dehydrogenase activity assay kit. (b) Culture supernatant was collected from the SSE-treated cells. Leptin production was determined by ELISA by subtracting the value measured at 450 nm using a mouse leptin immunoassay kit (R&D Systems). Data are presented as the mean ± SEM. compared with the differentiated control.