Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 4

Research Article

Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK1/2 in 3T3-L1 Cells

Figure 4

Effects of SSE on mRNA expression of lipid metabolism-related genes in 3T3-L1 adipocytes. (a) 3T3-L1 preadipocytes were differentiated into adipocytes by incubation with isobutylmethylxanthine, dexamethasone, and insulin (MDI) for 6 days. The cells were exposed to various concentrations of SSE (0, 25, 50, 100, 200, or 400 μg/mL) during the differentiation period. Total RNA was isolated and subjected to real-time RT-PCR for PPAR-γ (a) and C/EBP-α (b), FAS (c), LPL (d), and FABP4 (e). β-actin was used as a housekeeping gene.