Neutrophilic Lung Inflammation Suppressed by Picroside II Is Associated with TGF-β Signaling

Figure 2

PIC II is not an effective suppressor of NF-κB or activator of Nrf2. (a) RAW 264.7 cells were treated with indicated amounts of PIC II for 16 h and subsequently 15 min or 30 min with LPS. NF-κB (p65 RelA) was measured by Western blot. The membrane was stripped and reprobed with α-Lamin A/C antibody for ensuring an equal loading of nuclear proteins. (b) The band intensity of each band was analyzed by ImageJ, and the relative intensity of p65 RelA was calculated over Lamin A/C. Results are representatives of at least three independent experiments. Data are shown as the mean ± SEM of three measurements. was less than 0.05. (c) RAW 264.7 cells were treated with the indicated amounts of PIC II for 16 h along with sulforaphane (SFN, 4 h at 5 μM) and Nrf2 was analyzed by Western blot. (d) Similar to (b), the relative intensity of Nrf2 was calculated over Lamin A/C by ImageJ. No statistical significance was found.

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