Neutrophilic Lung Inflammation Suppressed by Picroside II Is Associated with TGF-β Signaling

Figure 6

PIC II treatment decreases the expression of proinflammatory cytokine gene expression in the lungs of ALI mice. (a) Total RNA extracted from the lung tissues of ALI mice () was analyzed by semiquantitative RT-PCR for the expression of representative proinflammatory cytokine genes. Two representatives in each group are shown. (b) Each band was quantitated by ImageJ, and relative expressions of those cytokine genes were calculated over a house-keeping gene, GAPDH. Data represent the mean ± SEM of each group in three measurements. and were less than 0.05 and 0.001, respectively, compared with the only LPS treated.

(a)

(b)