Evaluation of the Effects of a Combination of Japanese Honey and Hydrocolloid Dressing on Cutaneous Wound Healing in Male Mice

Figure 2

Macroscopic observations. (a) HE staining on day 3. The red arrows indicate the wound edges, and the green arrows indicate the edge of the new epithelium (bar, 200 m). (b) Azan staining on day 7. The white box indicates the wound area; that is, it surrounds both wound edges, the surface of the wound, and the bottom of the wound (bar, 200 m). (c) Immunohistological staining of myofibroblasts on day 14. Myofibroblasts were stained with anti- SMA antibody. The white box indicates the wound area; that is, it surrounds both wound edges, the surface of the wound, and the bottom of the wound (bar, 500 m). (d) Immunohistological staining of neutrophils on day 3. Neutrophils (arrows) were stained with anti-neutrophil antibody (bar, 20 m). (e) Immunohistological staining of macrophages on day 3. Macrophages (arrows) were stained with anti-Mac-3 antibody (bar, 20 m). D: dermis; G: granulation tissue.

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