The Inhibitory Effect of Kakkonto, Japanese Traditional (Kampo) Medicine, on Brain Penetration of Oseltamivir Carboxylate in Mice with Reduced Blood-Brain Barrier Function

Table 1

The measurement conditions of maker compound for quality control in kakkonto and maoto formulations.

Maker compound for QC	Column	Column temperature (°C)	Mobile phase	Flow rate (mL/min)	Wavelength (nm)	Internal standard solution (final concentration)
GA	TSK-gel ODS-80TM; (4.6 × 250 mm)	40	200 L/L phosphoric acid : acetonitrile (66 : 34)	1.5	254	30 g/mL amyl 4-hydroxybenzoate
PA			Deionized water : acetonitrile : phosphoric acid (850 : 150 : 1)	1.2	232	30 g/mL methyl 4-hydroxybenzoate
AM			50 mM potassium dihydrogen phosphate : methanol (5 : 1)	1.2	210	3 g/mL methyl 4-hydroxybenzoate