Xanthohumol Induces Growth Inhibition and Apoptosis in Ca Ski Human Cervical Cancer Cells

Figure 2

Xanthohumol reduced Ca Ski cell proliferation and induced apoptosis. (a) Dose response curve of the percentage of inhibition on the cells against different concentrations of xanthohumol (0–282 μM, equivalent to 0–100 μg/mL) following 24-hour, 48-hour, and 72-hour treatment. (b) The effects of xanthohumol on apoptosis by annexin V-FITC/PI staining. The 1st quadrant (lower left-LL) depicts percentage of viable cells, the 2nd quadrant (lower right-LR) depicts % of early apoptotic cells, the 3rd quadrant (upper right-UR) depicts late apoptotic or secondary necrotic cells, and the 4th quadrant (upper left-UL) depicts primary necrotic cells. Xanthohumol increased the percentage of late apoptotic or secondary necrotic cells (in UR quadrant).

(a)

(b)