Evaluation of Antifungal Activity and Mode of Action of New Coumarin Derivative, 7-Hydroxy-6-nitro-2H-1-benzopyran-2-one, against Aspergillus spp.

Table 4

MIC of Antifungal drugs and effect of combination with coumarin derivative, 7-hydroxy-6-nitro-2H-1-benzopyran-2-one (Cou-UNO₂), against A. flavus, ATCC 16013.

Antifungal + Cou-UNO2	MIC	FIC index
	(μg/mL)	(Type of interaction)
Cou-UNO2	16
Amphotericin B	2
Fluconazole	256
Itraconazole	128
Voriconazole	4
Cou-UNO2/Amphotericin B	16/2	2 (Indifferent)
Cou-UNO2/Fluconazole	16/256	2 (Indifferent)
Cou-UNO2/Itraconazole	8/32	0,75 (Additivity)
Cou-UNO2/Voriconazole	8/1	0,75 (Additivity)

Note. Cou-UNO₂: 7-hydroxy-6-nitro-2H-1-benzopyran-2-one. FIC: fractional inhibitory concentration. MIC: minimal inhibitory concentration.