Tongxinluo Prevents Endothelial Dysfunction Induced by Homocysteine Thiolactone In Vivo via Suppression of Oxidative Stress

Figure 2

Inhibition of PPARγ abolished TXL-suppressed oxidative stress and maintains cell viability in HTL-treated cells. Cultured HUVECs were pretreated with TXL (200 μg/mL) for 1 hour and then incubated with HTL (1 mM) for 24 hours in presence of GW9662 (0.01 mM). Cell was subjected to assay: (a) cell viability by MTT and (b) intracellular ROS productions by DHE fluorescence. (c) Quantitative analysis of ROS productions. All data were expressed as mean ± SEM. is 3 in each group. versus Con, versus HTL alone, and versus HTL + TXL.

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