The Anti-Inflammatory Effect of Prunus yedoensis Bark Extract on Adipose Tissue in Diet-Induced Obese Mice

Figure 3

Effect of PY extract and its fractions on PMA-stimulated cell viability and scavenger receptor gene expression. ((a)–(d)) THP-1 cells were cultured with PMA (100 nM) for 24 h and 48 h. PY extract (a) and its aqueous (W), ethyl acetate (EA), and chloroform (CH) fractions were added into the media simultaneously with PMA. Cell viability was determined by reading the amount of colored formazan. (e) THP-1 cells were stimulated with PMA for 48 h, and PY extract (200 μg/mL) or fractions (W: 100 μg/mL, EA: 100 μg/mL, CH: 10 μg/mL) thereof were added into the media simultaneously with PMA. Gene expression levels were determined using qPCR. Target gene expression was normalized to that of actin. Data are expressed as means ± SD of three independent experiments. (−) PMA negative. , , and versus cells treated with PMA only (control).

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