Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress

Figure 9

Protective effects of FFBJ and FFOJ on DNA damage induced by H₂O₂. (a) Levels of 8-oxo-dG are measured as emission signals of fluorochrome FITC-labeled avidin collected in the FL-1 channel. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. versus control cultures and versus H₂O₂-treated cells. (b) Images of comet assay captured by fluorescence microscopy at a magnification of 400x. A representative experiment that was replicated three times with similar results is shown. Percentages of tail DNA (TDNA) are indicated.

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