Induction of Thioredoxin Reductase 1 by Korean Red Ginseng Water Extract Regulates Cytoprotective Effects on Human Endothelial Cells

Figure 1

Effect of KRG on TrxR1 expression in HUVECs. Cells were treated with the indicated concentrations of KRG for 18 h and then the protein in cell lysates was analyzed by Western blot using TrxR1 specific antibody (a). RT-PCR was performed to measure the levels of TrxR1 mRNA transcript (c). Cells treated with 1 mg/mL KRG were harvested at various time intervals for analysis (b and d). The level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control.

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