Evidence-Based Complementary and Alternative Medicine

Review Article

Recent Advance in Applications of Proteomics Technologies on Traditional Chinese Medicine Research

Table 1

Proteomics applied in the effective mechanisms of TCM treatments in vitro.
DiseasesCHF, CHM, and CHM compoundTargets or signaling pathwaysProteomics methodsReferences
Cancers    
 Cervical carcinoma9,11-Dehydroergosterol peroxideStathmin 1MALDI-TOF MS/MS[27]
 Cervical carcinomaTriterpenesIL-17E, eIF5A, peroxiredoxin-2, and ubiquilin-22DE, MALDI-TOF MS/MS[28]
 Cervical carcinomaTanshinone IIAVimentin, Maspin, a- and b-tubulin, and GRP752DE, MALDI-TOF MS[29]
 Cervical carcinomaTanshinone IIAEndoplasmic reticulum stress pathways2DE, MALDI-TOF-TOF MS[30]
 Hepatocellular carcinoma1,3,6,7-Tetrahydroxyxanthoneβ-tubulin, 14-3-3σ, and P162DE, MALDI-TOF-MS, MS/MS[31]
 Hepatocellular carcinoma1,3,5-TrihydroxyxanthoneHSP272DE, MALDI-TOF-TOF MS[32]
 Colorectal carcinomaBaicaleinPeroxiredoxin-62DE, MALDI-TOF-TOF MS[33]
 Colon carcinomaTriptolide14-3-3ξ2DE, MALDI-TOF-TOF MS[34]
 Gastric adenocarcinomaCelastrus orbiculatusHSP27, NF-κB/Snail signal pathways2DE, MALDI-TOF-TOF MS[15]
 Gastric adenocarcinomaTanshinone IIAp53, AKT, G6PI, and LDHBiTRAQ[35]
 Breast carcinomaCurcuminTDP-43, SF2/ASF, eIF3i, 3-PGDH, and ERP292DE, MALDI-TOF MS[14]
 Breast carcinomaCoptis chinensis Franch.ROS generation2D-DIGE, MS[36]
 LeukemiaTriterpenesAldolase A, GAPDH, and HSP90-Alpha2DE, MALDI-TOF-MS[37]
Cardiocerebrovascular diseases    
 Heart diseaseShuanglong formulaEnergy metabolism2DE[38]
 Cardiovascular diseaseSalvianolic acid BHSP27 and mitofilin2DE, MALDI-TOF-MS/MS[39]
 Alzheimer’s diseaseHuperzine ATrp53LC-MS/MS[40]
 Parkinson’s diseaseAcanthopanax senticosus HarmsLewy body, mitochondrial energy metabolismiTRAQ[41]
 Neurodegenerative disordersTianmaCALR, FKBP3/4, HSP70/90, and AIP5iTRAQ[42]
 Neurodegenerative disordersTianmaNxn, Dbnl, Mobkl3, Clic4, Mki67, and BaxiTRAQ[43]
Inflammatory diseases    
 Inflammatory diseasesBi-qi capsuleiNOS, COX-2, TNF-α, IL-6, and IL-1βProteome profiler array[44]
 Inflammatory diseasesZuojin pilliNOS, COX-2, IL-6, IL-1β, TNF-α, and NF-κBProteome profiler array[45]
 NeuroinflammationAcanthopanax senticosus extractNitrosative stress pathway2D-DIGE, LC-ESI-MS/MS[12]
Other diseases    
 Wound healingLithospermi radixAntioxidant activity, antiapoptosis activity2DE, LC-MS/MS[46]
 Oxidative damageIsopsoralenProteins (m/z 6532 and m/z 6809)SELDI-TOF-MS[47]
 Ischemia reperfusion injuryTao Hong Si Wu decoctionNrf2-mediated phase II enzymes2DE, MALDI-TOF MS[48]
 PostcataractsCurcuminProteins (m/z of 8093 and 13767)MS[49]