Evaluation of the Isoflavone Genistein as Reversible Human Monoamine Oxidase-A and -B Inhibitor

Figure 4

Genistein (GST) inhibited human monoamine oxidases (hMAO-A and hMAO-B isozymes) H₂O₂ byproduct formation differently () by using tyramine as a substrate (a). The enzymatic reaction was monitored in hMAO-A (b) and hMAO-B (c) without GST (dotted line) or in the presence of different GST concentrations at RT. IC₅₀s and data points were presented as the mean ± SEM, . RS is relative selectivity to inhibit B isozyme. The significance of difference of RS against hMAO-B was determined using unpaired -test.

(a)

(b)

(c)