Clinical Effect and Mechanism of Yisui Shengxue Granules in Thalassemia Patients with Mild, Moderate, or Severe Anemia

Figure 9

Benzidine staining to identification of erythroid differentiation. Benzidine staining positive cells were where the white arrows indicate, cells present with blue. Colonies were collected from a well using a blunt-ended needle, resuspended with PBS, and then stained with benzidine. Cells were observed under an inverted phase contrast microscope after 10 min. (a) Cells before benzidine staining. (b) Benzidine staining positive cells after staining (40x). (c) Benzidine staining positive cells after staining (100x). (d) Benzidine staining positive cells after staining (100x).

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