Hataedock Treatment Has Preventive Therapeutic Effects in Atopic Dermatitis-Induced NC/Nga Mice under High-Fat Diet Conditions

Figure 5

The regulation of mast cells activation. The distribution of degranulated mast cell (vacant arrow) in dermal papillae was increased in the HDE group but decreased in the HTT group (Luna’s method; bar size, 50 μm). The Substance P positive reaction (arrow indicates dark brown) in HTT significantly decreased (Substance P immunohistochemistry; bar size, 50 μm). The MMP-9 positive reaction (arrow indicates dark brown) in HTT remarkably decreased (MMP-9 immunohistochemistry; bar size, 50 μm). Data of Substance P and MMP-9 image analysis was also showing the same result (). Ctrl: normal feeding, HF: high-fat diet, HDE: high-fat diet and untreated AD-induced, HTT: high fat diet and Hataedock treated AD-induced, EP: epidermis, DE: dermis, and MMP-9: matrix metalloproteinases-9. , compared with the Ctrl group; , compared with the HF group; , compared with the HDE group.