Research Article

Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima

Table 3

Effect of nitrogen concentrations on L-asparaginase production by Spirulina maxima.

Nitrogen concentrations as NaNO3Enzyme activityProteinSpecific activityYield of enzyme
IUmg/gIU/mgIU/L

1.25 g/L356 ± 6.2212 ± 3.51.67 ± 0.2132.39 ± 2.6
2.5 g/L control594 ± 6.5332 ± 5.21.79 ± 0.1143.86 ± 2.5
5.0 g/L898 ± 11.6405 ± 6.52.21 ± 0.2251.28 ± 3.4

Mean values of three replicates, ±SD.