Research Article
Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima
Table 3
Effect of nitrogen concentrations on L-asparaginase production by Spirulina maxima.
| Nitrogen concentrations as NaNO3 | Enzyme activity | Protein | Specific activity | Yield of enzyme | IU | mg/g | IU/mg | IU/L |
| 1.25 g/L | 356 ± 6.2 | 212 ± 3.5 | 1.67 ± 0.21 | 32.39 ± 2.6 | 2.5 g/L control | 594 ± 6.5 | 332 ± 5.2 | 1.79 ± 0.11 | 43.86 ± 2.5 | 5.0 g/L | 898 ± 11.6 | 405 ± 6.5 | 2.21 ± 0.22 | 51.28 ± 3.4 |
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Mean values of three replicates, ±SD.
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