Sex-Dependent Effects of Dietary Genistein on Echocardiographic Profile and Cardiac GLUT4 Signaling in Mice

Figure 4

Effect of genistein treatment on total cardiac Akt, AMPK, SYNIP, and MEF2A protein expression in intact male and female mice. (a) AMPK. Typical western blot demonstrating AMPK and GAPDH expression in cardiac tissue from two samples each of 600 G- and 0 G-treated male and female mice. Average AMPK/GAPDH ratio is shown comparing 600 G- and 0 G-fed male and female mice (/group). AMPK and GAPDH bands were observed at 62 kDa and 37 kDa, respectively. (b) Akt. Typical western blot demonstrating Akt and GAPDH expression in cardiac tissue from two samples each of 600 G- and 0 G-treated male and female mice. Average Akt/GAPDH ratio is shown comparing 600 G- and 0 G-fed male and female mice (/group). Akt and GAPDH bands were observed at 60 kDa and 37 kDa, respectively. (c) SYNIP. Typical western blot demonstrating SYNIP and GAPDH expression in cardiac tissue from two samples each of 600 G- and 0 G-treated male and female mice. Average SYNIP/GAPDH ratio is shown comparing 600 G- and 0 G-fed male and female mice (/group). SYNIP and GAPDH bands were observed at 62 kDa and 37 kDa, respectively. (d) MEF2A. Typical western blot demonstrating MEF2A and GAPDH expression in cardiac tissue from two samples each of 600 G- and 0 G-treated male and female mice. Average MEF2A/GAPDH ratio is shown comparing 600 G- and 0 G-fed male and female mice (/group). MEF2A and GAPDH bands were observed at 55 kDa and 37 kDa, respectively. Values are reported as mean ± SD. 0 GF: genistein-free fed females; 0 GM: genistein-free fed males; 600 GF: 600 mg genistein/kg diet-fed females; 600 GM: 600 mg genistein/kg diet-fed males. The genistein-free fed groups (0 G) are represented by open bars, and genistein-fed groups (600 G) are represented by the solid bars.

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