Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer’s Disease

Figure 2

The herbal formula B401 effectively inhibits glutamate-induced excitotoxicity, H₂O₂-induced oxidative stress, and AChE activity in Tet-On Aβ₄₂-GFP SH-SY5Y cells. (a) (A) Tet-On Aβ₄₂-GFP SH-SY5Y cell viability was measured after 24 hours of glutamate-treatment (Glu, 100 mM). The herbal formulas B401 (10–80 mg/mL) and MK-801 (10 μM) were added individually in the presence of glutamate. (B) Tet-On Aβ₄₂-GFP SH-SY5Y cell viability was measured after 24 hours of hydrogen peroxide treatment (H₂O₂, 200 μM). The herbal formulas B401 (10–80 mg/mL) and MK-801 (10 μM) were added individually in the presence of H₂O₂. (b) (A) AChE activity of Tet-On Aβ₄₂-GFP SH-SY5Y cells was analyzed after treating with 10 μg/mL Dox in the absence or presence of the herbal formula B401 at indicated doses. (B) AChE activity of SH-SY5Y cells was analyzed in the absence or presence of the herbal formula B401 at indicated doses. Results were shown as mean ± SEM (, two-way ANOVA followed by a Student-Newman-Keuls multiple comparisons post test), and the number of experiments was six for each treatment.

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