Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer’s Disease

Figure 6

Oral B401 treatment effectively reduces blood ROS and enhances SOD2 but decreases 3-NT expressions in the brain of 3× Tg-AD mice. (a) (A) Blood ROS corresponding to the brain in 3× Tg-AD mice and their control (non-AD mice) by using chemiluminescence analysis. (B) Quantified blood ROS in 3× Tg-AD mice with oral B401 treatment were significantly decreased compared to those 3× Tg-AD mice with sham treatment but were greater compared to their control. (b) IHC staining shows SOD2 expression (marked by arrows) and (c) 3-NT expression (marked by arrows) in CA1 and dentate gyrus areas of 3× Tg-AD mice and their control (non-AD mice). Scale bars: 30 μm. (d) Western blotting analysis shows the following. (A) Whole brain SOD2 and 3-NT expression levels of 3× Tg-AD mice and their control. (B) Quantified brain SOD2 levels of the 3× Tg-AD mice with oral B401 treatment were significantly increased, but brain 3-NT levels were significantly decreased compared to those 3× Tg-AD mice with sham treatment. Results were shown as mean ± SEM ( < 0.05; , one-way ANOVA followed by a Student-Newman-Keuls multiple comparisons post test), and the number of experiments was six for each treatment.

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