Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer’s Disease

Figure 8

Oral B401 treatment effectively inhibits brain p-Tau expression in 3× Tg-AD mice. (a) IHC staining shows p-Tau expression (marked by arrows) in hippocampal CA1 and dentate gyrus (DG) areas of 3× Tg-AD mice and their control (non-AD mice). Scale bars: 30 μm. (b) Western blotting analysis shows the following. (A) Whole brain p-Tau expression levels of 3× Tg-AD mice and their control. (B) Quantified brain p-Tau levels of 3× Tg-AD mice with oral B401 treatment were significantly less than those 3× Tg-AD mice with sham treatment but were significantly greater than their control. Results were shown as mean ± SEM (; , one-way ANOVA followed by a Student-Newman-Keuls multiple comparisons post test), and the number of experiments was six for each treatment. AD: Alzheimer’s disease; CA1: region 1 of hippocampus proper; DG: dentate gyrus; IHC: immunohistochemistry; SEM: standard error of the mean.

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