The Extract of Fructus Psoraleae Promotes Viability and Cartilaginous Formation of Rat Chondrocytes In Vitro

Figure 4

The effect of P-e on chondrocytes ECM gene expression and cartilaginous formation. (a) Gene expression level of different cartilaginous-related genes: collagen II, aggrecan, and Sox-9 at days 3 and 6 with P-e treatments. The data were analyzed by the relative quantification method (mean sd, ). versus DMSO group (control) was accepted as statistically significant. (b) Collagen II immunofluorescent staining of chondrocytes with P-e treatment for 1-week culture. (c) H&E and alcian blue staining of cartilaginous tissue sections of the pellet with P-e conditioned culturing for 10 days.

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