Preparative Purification of Bioactive Compounds from<i> Flos Chrysanthemi Indici</i> and Evaluation of Its Antiosteoporosis Effect

<div>Effect of four compounds on osteogenesis-related gene and protein expression. (a) MC3T3-E1 cells were pretreated with 1 <i>μ</i>M test agent and were then induced to undergo osteogenesis for 7 or 10 days. Levels of t type I collagen (COL-I), osteopontin (OPN), osteocalcin (OCN), and runt-related transcription factor 2 (Runx2) mRNA expression were analyzed by RT-qPCR. (b) Western blot analysis of AKT phosphorylation and Runx2 expression after treatment of MC3T3-E1 cells with the indicated concentration of test agent. 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Evidence-Based Complementary and Alternative Medicine

fig9

Figure 9

Figure 9: Preparative Purification of Bioactive Compounds from<i> Flos Chrysanthemi Indici</i> and Evaluation of Its Antiosteoporosis Effect 

Evidence-Based Complementary and Alternative Medicine

Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Figure 9