Soshiho-Tang Aqueous Extract Exerts Antiobesity Effects in High Fat Diet-Fed Mice and Inhibits Adipogenesis in 3T3-L1 Adipocytes

Figure 4

Inhibitory effect of SST extract on TG production in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes by incubation with isobutylmethylxanthine, dexamethasone, and insulin (MDI) for 8 days. The cells were treated with or without SST or GW9662 (20 μM) during the differentiation period. (a) Lipid accumulation in the cells was analyzed by Oil-Red O staining. (b) The TG content was measured enzymatically using a commercial kit. (c) Culture supernatant was collected from the SST-treated cells. Leptin production was determined by ELISA by using a mouse leptin immunoassay kit. Data are presented as mean ± SEM. and versus differentiated cells. GW9662 (20 μM) was used as a positive control.

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