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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 2758140, 9 pages
http://dx.doi.org/10.1155/2016/2758140
Research Article

Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide-Induced Inflammation through Suppression of NLRP3/Caspase-1 Inflammasome in Human Peripheral Blood Mononuclear Cells

1Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China
2Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun 130021, China
3School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China

Received 2 March 2016; Revised 30 June 2016; Accepted 13 July 2016

Academic Editor: Hyunsu Bae

Copyright © 2016 Jingying Sai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Yinhua Miyanling Tablet (YMT), the Chinese formula, has long been administrated in clinical practice for the treatment of acute pyelonephritis and acute urocystitis. In the current study, we aimed to investigate the anti-inflammatory effect of YMT in vitro and to evaluate the association between anti-inflammation and innate immune response. Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density gradient centrifugation and then were stimulated by Lipopolysaccharide (LPS). The differential gene expression of inflammation-related genes after drug administration was assessed using PCR array, and the protein levels of differential genes were measured by ELISA and Western blot. The result showed that YMT significantly inhibited the expression of NLRP3, Caspase-1, and the downstream cytokine IL-1β and suppressed the production of inflammatory mediators TNF-α, IL-6, IL-10, and MCP-1 in a dose-dependent manner compared to the LPS group . The finding indicated that YMT exhibited anti-inflammatory effect in vitro by suppressing the NLRP3/Caspase-1 inflammasome, and that may have therapeutic potential for the treatment of inflammatory diseases.